Comparison of sperm subpopulation structures in first and second ejaculated          semen from Japanese black bulls by a cluster analysis of sperm motility evaluated by a          CASA system

Chihiro KANNO; Kentaro Q. SAKAMOTO; Yojiro YANAGAWA; Yoshiyuki TAKAHASHI; Seiji KATAGIRI; Masashi NAGANO

doi:10.1292/jvms.17-0012

Theriogenology

Comparison of sperm subpopulation structures in first and second ejaculated semen from Japanese black bulls by a cluster analysis of sperm motility evaluated by a CASA system

Chihiro KANNO, Kentaro Q. SAKAMOTO, Yojiro YANAGAWA, Yoshiyuki TAKAHASHI, Seiji KATAGIRI, Masashi NAGANO

Author information

Chihiro KANNO
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Kentaro Q. SAKAMOTO
Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Yojiro YANAGAWA
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Yoshiyuki TAKAHASHI
Genetics Hokkaido Association, Kita-4, Nishi-1, Kita-ku, Sapporo 060-0004, Japan
Seiji KATAGIRI
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Masashi NAGANO
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan

Keywords: bovine, cluster analysis, computer-assisted sperm analysis, motility, spermatozoa

JOURNAL FREE ACCESS

2017 Volume 79 Issue 8 Pages 1359-1365

DOI https://doi.org/10.1292/jvms.17-0012

Browse “Advance online publication” version

Details

Abstract

In the present study, bull sperm in the first and second ejaculates were divided into subpopulations based on their motility characteristics using a cluster analysis of data from computer-assisted sperm motility analysis (CASA). Semen samples were collected from 4 Japanese black bulls. Data from 9,228 motile sperm were classified into 4 clusters; 1) very rapid and progressively motile sperm, 2) rapid and circularly motile sperm with widely moving heads, 3) moderately motile sperm with heads moving frequently in a short length, and 4) poorly motile sperm. The percentage of cluster 1 varied between bulls. The first ejaculates had a higher proportion of cluster 2 and lower proportion of cluster 3 than the second ejaculates.

Corresponding author

Register with J-STAGE for free!