The precursor osteoblast-like cell, MC3T3-E1 cell line, enhances sodium–calcium exchanger 1 (Ncx1) gene expression by stretch stimuli prior to osteoblast differentiation

抄録

This study evaluated the expression of genes involved in the concentration of Ca²⁺ in precursor osteoblast-like cell, MC3T3-E1 subjected to stretching stimuli. Transient receptor potential vanilloid 4 (Trpv4) gene expression, the factor that is activated by stretch stimulation and enables inflow of Ca²⁺ from the extracellular space, was not affected as a result of stretch stimulation; conversely, the expression of sodium-calcium exchanger 1 (Ncx1) gene involved in outflow of intracellular Ca²⁺ increased, depending on stimulation intensity. Localization of Ca²⁺ correlated with the positioning of the endoplasmic reticulum, and intracellular Ca²⁺ decreased in inverse proportion to the intensity of the stretching force. These results suggest that stretch stimulation activates intracellular Ca²⁺ elimination rather than Ca²⁺ uptake before osteoblast differentiation.