Estimation of prion infectivity in tissues of cattle infected with atypical          BSE by real time-quaking induced conversion assay

Kazuhei SAWADA; Akio SUZUKI; Takeshi YAMASAKI; Yoshifumi IWAMARU; Yuichi MATSUURA; Kohtaro MIYAZAWA; Kentaro MASUJIN; Ryuichiro ATARASHI; Motohiro HORIUCHI

doi:10.1292/jvms.19-0003

Public Health

Estimation of prion infectivity in tissues of cattle infected with atypical BSE by real time-quaking induced conversion assay

Kazuhei SAWADA, Akio SUZUKI, Takeshi YAMASAKI, Yoshifumi IWAMARU, Yuichi MATSUURA, Kohtaro MIYAZAWA, Kentaro MASUJIN, Ryuichiro ATARASHI, Motohiro HORIUCHI

著者情報

Kazuhei SAWADA
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Hokkaido University, Kita18, Nish 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Akio SUZUKI
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Hokkaido University, Kita18, Nish 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Takeshi YAMASAKI
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Hokkaido University, Kita18, Nish 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Yoshifumi IWAMARU
Global Station for Zoonosis Control, Global Institute for Collaborative Research and Education, Hokkaido University, Kita 20, Nishi 10, Kita-ku, Hokkaido 001-0020, Japan
Yuichi MATSUURA
Prion Disease Research Center, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Kohtaro MIYAZAWA
Prion Disease Research Center, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Kentaro MASUJIN
Prion Disease Research Center, National Institute of Animal Health, 3-1-5, Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Ryuichiro ATARASHI
Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200, Kihara, Kiyotake-cho, Miyazaki, Miyazaki 889-1692, Japan
Motohiro HORIUCHI
Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Hokkaido University, Kita18, Nish 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan
Global Station for Zoonosis Control, Global Institute for Collaborative Research and Education, Hokkaido University, Kita 20, Nishi 10, Kita-ku, Hokkaido 001-0020, Japan

キーワード: atypical bovine spongiform encephalopathy, H-BSE, L-BSE, prion, RT-QuIC

ジャーナルフリー

2019 年 81 巻 6 号 p. 846-850

DOI https://doi.org/10.1292/jvms.19-0003

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詳細

抄録

Atypical bovine spongiform encephalopathy (BSE), first identified in 2004, poses a threat due to the potential to spread the disease to cattle and other animals, including humans. Here, we estimated prion titers in various tissues of cattle infected with atypical BSE using a real-time quaking-induced conversion assay that detects amyloid seeding activity of a disease-specific prion protein, PrP^Sc, a major component of prions. PrP^Sc was detected both in and outside of nerve tissues, and some of the peripheral nerve tissues contained relatively high prion titers. Low titers of prions were also observed in masseter, jejunum, and adrenal glands. Quantitative data on prion infectivity in tissues of atypical BSE-affected cattle is useful to assess the risk of atypical BSE.

責任著者(Corresponding author)

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