Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis
Zhang QUANTan HAIMINGCai XIAOYAOYuan WEIFENGJia HONGZhu HONGFEI
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ジャーナル フリー 早期公開

論文ID: 15-0216

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A multiplex PCR (m-PCR) with primers targeting the 16S rRNA, Rv3873, and a 12.7-kb fragment in the genomes of a Mycobacterium tuberculosis complex was designed for the differential diagnosis of M. tuberculosis, M. bovis, M. bovis BCG, and non-tuberculosis Mycobacterium (NTM). The specificity of this assay was 100% and the detection limit was 15 pg of genomic DNA. Of the 206 blinded clinical samples, the detection rate of M. bovis infection by m-PCR was lower than that of the interferon gamma (IFN-γ) release assay; however, the false-positive rate by the tuberculin skin test and false-negative samples in the IFN-γ release assay were reduced. Our findings indicated that our m-PCR method is a useful tool for complementation to differentiate M. bovis from M. tuberculosis and NTM species.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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