Molecular characterization of an Akabane virus isolate from West Java, Indonesia

Suryo PURNOMO EDI; Afif IBRAHIM; Rinto SUKOCO; Lukman BUNALI; Masaji TAGUCHI; Tomoko KATO; Tohru YANASE; Hiroaki SHIRAFUJI

doi:10.1292/jvms.17-0009

This article has now been updated. Please use the final version.

Molecular characterization of an Akabane virus isolate from West Java, Indonesia

Suryo PURNOMO EDI, Afif IBRAHIM, Rinto SUKOCO, Lukman BUNALI, Masaji TAGUCHI, Tomoko KATO, Tohru YANASE, Hiroaki SHIRAFUJI

Author information

Suryo PURNOMO EDI
Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Indonesia
Afif IBRAHIM
Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Indonesia
Rinto SUKOCO
Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Indonesia
Lukman BUNALI
Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Indonesia
Masaji TAGUCHI
Disease Investigation Center Subang, Directorate General of Livestock and Animal Health Services, Indonesia
Tomoko KATO
Kyushu Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Tohru YANASE
Kyushu Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization
Hiroaki SHIRAFUJI
Kyushu Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization

Keywords: Akabane virus, arbovirus, cattle, genogroup

JOURNAL FREE ACCESS Advance online publication

Article ID: 17-0009

DOI https://doi.org/10.1292/jvms.17-0009

The final version of this article is now available: Vol. 79 (2017), No. 4 pp. 765-770

Details

Abstract

We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.

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