Immunohistochemical localization of inhibin/activin subunits in adult Asian elephant (Elephas maximus) testes

Sirinart CHAICHANATHONG; Kasuyoshi TAYA; Gen WATANABE; Kentaro NAGAOKA; Worawidh WAJJWALKU; Apichaya SUDSUKH; Nikorn THONGTIP

doi:10.1292/jvms.17-0531

This article has now been updated. Please use the final version.

Immunohistochemical localization of inhibin/activin subunits in adult Asian elephant (Elephas maximus) testes

Sirinart CHAICHANATHONG, Kasuyoshi TAYA, Gen WATANABE, Kentaro NAGAOKA, Worawidh WAJJWALKU, Apichaya SUDSUKH, Nikorn THONGTIP

Author information

Sirinart CHAICHANATHONG
Center for Agricultural Biotechnology, Kasetsart University
Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)
Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen campus
Kasuyoshi TAYA
Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Gen WATANABE
Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Cooperative Division of Veterinary Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology
Kentaro NAGAOKA
Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Cooperative Division of Veterinary Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology
Worawidh WAJJWALKU
Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen campus
Apichaya SUDSUKH
Department of Obstetrics, Gynecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University
Nikorn THONGTIP
Center for Agricultural Biotechnology, Kasetsart University
Department of Large Animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University

Keywords: Asian elephant, immunohistochemistry, inhibin/activin subunits, Leydig cells, Sertoli cells

JOURNAL FREE ACCESS Advance online publication

Article ID: 17-0531

DOI https://doi.org/10.1292/jvms.17-0531

The final version of this article is now available: Vol. 80 (2018), No. 3 pp. 549-552

Details

Abstract

Immunolocalization of inhibin-α and inhibin/activin βA and βB subunits in the testes of Asian elephant was determined. Testicular sections were immunostained with polyclonal antisera against inhibin subunit-α and inhibin/activin βA and βB using the avidin-biotin-peroxidase complex method. Positive immunostaining against inhibin-α subunit was strongly present in Sertoli cells, and positive immunostaining for the inhibin/activin βA and βB subunits was observed in both Sertoli and Leydig cells. These results indicated that while Sertoli cells are the predominant source of inhibin and activin secretions in the testes of adult male Asian elephant, Leydig cells are a source of activin but not inhibin.

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