Abstract
The measuring method for the free acidic suostance and the free basie substance inanimal meat, can be simply performed by appling the method of titration in non aqueoussolutions, and the method of chelate. Procedure and results of these measuring methodswere as follows :( I ) Tlre measuring rrnetlxod for the free acidic substancea. Benzen-alcohol solvents were added to a fixed quantity of tlxe rmaterial.b. After 3 -5 minutes, it was titrated witlt methanol KOI-I.c. The result of titration XV2[S indicated as acetic acid, just as in tlne case of general foodanalysis. The quantity of the acetic acid (S) can be calculated by the following formula.( 2 ) The measuring method for the free basic substancea. Glycol-alcohol solvents NVCFC added to a fixed quantity of the material.b. After 5 - 10 minutes, titration was performed by HCI0j.c. The result of" the titration was indicated as NU. in the following formula.Nll., ?? - ]-3401.X (A .....B)F X..1.00. .. (A. -B).1"34.0 lWW(3 ) The measuring method for the free fatty acida. 75% methanol was added to a fixed quantity of the material, after it was neutralizedwith a dilution of KOH, then it was boiled until it dried up.b. Calcium chloride Solution of known density was added, and then the rernainder ofthe calcium was titrated with 2 Na-E.D.T.A. solution.c. The result of the analysis was indicated as caproic acid in the following formula.C..., 7 .....0.-4O.OQ.X-(A-.-.......B)F X (10.0.)f.. (A B)F- 155.35 - -25.8 x WW
