抄録
In regard to the histopathological changes in the case of infectious anemia, in horses, at has been recognized that the active changes in the reticulo-endothelial system, thatis, the swelling, the proliferation and the hemosiderosis of the R.E.S. are observed togetherwith great destruction of the red corpuscles, especially in the liver and spleen etc..""It was found by means of our new complement fixation test" that the ferritin whichwas originally the storage iron in the reticulo-endothelial system, was contained in thesera of the jugular veins of horses having infectious anemia.It may be considered that such observation of the free ferritin in the sera meansthe degeneration and the collapse of the reticulo-endothelial system, mainly in the spleen, next in the liver and the bone marrow.In this report, from such a consideration, we made observations on the correlation.among the complement fixation tests on free ferritin in tlte sera, the histopathologicalchanges and the progress of the disease, mainly in the case of infectious anemia inhorses.lhe results are summarized as follows :I) Both, in the horses from the slaughter-house, having infectious anemia, and inthe artificially anemia-infected horses, it was found that the prosperity and decline ofthe free ferritin was very closely related to the histopathological changes in the reticulo-.endothelial system, especially in the liver and spleen, that is, the swelling and prolife-ration of the R.E.S., the appearance of siderocytes and lymphoid cells, and the distensionof capillaries. Cf. Table I & Table 2.2) In the artificially anemia-infected horses, the complement fixation tests on thefree ferritin were negative during the incubation period after the inoculation of equineinfectious anerruia virus, but the complement fixation tests vvere changed into the positivereaction without exception as soon as the horses had an attack of fever due to theactivities of the virus and carrte to indicate rapidly higher" positive reactions with febrilerises of the temperatture. Theru, the reactiouts became weak with comparative rapiditywith the fall of the temperature, but indicated weak positive reactions continuously fora comparatively long period after the fall of the temperature.Furthermore, it was found that the prosperity and dec[ir?e of free ferritin in thecase of infectious anemia in horses followed the same tetrden?cies as the slightness andseriousness of the clinical changes in the diseased horses. Cf. Table 3, Fig. l, Fig. 2& Fig. 3.3) The free ferritin in the serum was observed and comfirmecl as the crystal bycentrifuga, tion at 40000 r.p.m. -105500 g for one hour and the addition of CdS0) for theC.F.T.-positive substance in the serurn of the jugular vein of the artificially anemia-infectedhorse in the dura.tion of the febrile attacks. Cf. Fir. 3 & Plate II.4) The complement fixation tests on the free ferritin were negative irr almost allthe healthy horses that were genera, fly normal histopathologically. Cf. Table 4.From the above experimenta.l results, it is considered that the cornplerrternt fixationtest 011 the free ferritirt may become a method for the supplementary diagnosis of asyndrome that is accomparried by the swelling, the proliferation, the degeneration andthe collaps xxith hemosiderosis of the reticuro-endothelial system, especially, in the liverand spleen, avtd also that tlte complement fixation test may become a sensitive means bywhich we may be able to presume, indirPlate IIFig. 9. The liver of artificially are-mia-infected horse in the fever paroxysm (by the liver puncture).-T.U. 1.Free Ferritin C.F.T. 1:240, H.E.stain. X200Fig. 10. The liver of artificially anemia-infected horse immediately after the fever paroxysm (by theliwer puncture). -T.U. 1.Free Ferritin-C.F.T. 1:3, I4.E.stain. X200Fig. [the rest omitted]
