Comparative Study on Cross-Reaction of Leptospiral Antibodies in Several Serological Tests to Detect Antibodies to Borrelia burgdorferi in Dogs

Abstract

The effects by the leptospiral antibody are described at several serological tests for the detection of the antibody to B. burgdorferi in dogs. The titers at ELISA used with the ultrasonicated bacterial antigen (US-ELISA) were related with IFA. But cross reaction with the leptospiral antibody were shown in an agglutination test. Non-specific and cross reactions were observed in ELISA used with formalin-killed bacterial antigen. This indicates that US-ELISA is a reliable serodiagnosis method for Lyme disease in dogs in eliminating the cross reaction with leptospiral antibody.