1994 Volume 56 Issue 5 Pages 1001-1003
The stable expression of the alpha chain of the feline cytotoxic T cell differentiation antigen (fCD8α) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains the Rous sarcoma virus long terminal repeat and a neo resistant gene. After three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclonal antibody OKT8.