Stable Expression of the cDNA Encoding the Feline CD8α Gene

Marcelo Ricardo PECORARO; Yasushi KAWAGUCHI; Masatsugu OKITA; Yasuo INOSHIMA; Yukinobu TOHYA; Chieko KAI; Takeshi MIKAMI

doi:10.1292/jvms.56.1001

Marcelo Ricardo PECORARO, Yasushi KAWAGUCHI, Masatsugu OKITA, Yasuo INOSHIMA, Yukinobu TOHYA, Chieko KAI, Takeshi MIKAMI

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Keywords: feline CD8α, stable expression, T cell differentiation antigen

JOURNAL FREE ACCESS

1994 Volume 56 Issue 5 Pages 1001-1003

DOI https://doi.org/10.1292/jvms.56.1001

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Abstract

The stable expression of the alpha chain of the feline cytotoxic T cell differentiation antigen (fCD8α) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains the Rous sarcoma virus long terminal repeat and a neo resistant gene. After three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclonal antibody OKT8.

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