Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
Passage of Chicken Egg Yolk Antibody Treated with Hydroxypropyl Methylcellulose Phthalate in the Gastrointestinal Tract of Calves
Yutaka IKEMORIMasashi OHTAKouji UMEDARobert C. PERALTAMasahiko KUROKIHideaki YOKOYAMAYoshikatsu KODAMA
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1996 Volume 58 Issue 4 Pages 365-367

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Abstract

Two types of chicken egg yolk antibody samples for oral passage trials in calves were prepared; (1) hydroxypropyl methylcellulose phthalate (HPMCP) antibody powder (HAP) - a powder produced by spray-drying a supernatant obtained after precipitation of lipids from egg yolk with HPMCP and (2) control antibody powder (CAP) - a powder produced from an antibody solution without HPMCP. Antibody activity and pattern of distribution of both antibody preparations in the gastrointestinal tract of calves were compared by enzyme-linked immunosorbent assay. At 2 hr post administration, anti-K99 fimbrial antibodies from both the CAP and the HAP were detected in the abomasum of calves with titers of 1:128 and 1:256, respectively. However, at 4 hr, anti-K99 fimbrial titers of the CAP and the HAP were reduced to 1:2 and 1:64, respectively, due to digestion in the abomasum. These results indicated that the egg yolk antibody powder with HPMCP was more resistant against gastric juice in the stomach, thereby, ensuring a transfer of functional antibodies to the small intestine of calves after oral administration.

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