An Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Antibody against Streptococcus suis Type 2 in Infected Pigs

Abstract

An ELISA test for the detection of antibody against S.suis type 2 in pigs was developed and applied to field sera. The best sensitivity and specificity of ELISA were obtained when a purified polysaccharide antigen was used. It showed no cross reaction with sera against other serotypes of S.suis and other pathogenic bacteria. A total of 264 sera were collected from 20 pig farms and examined with the antibody against S.suis type 2. In the affected farms, 17.0% of pigs tested were positive, 9.8% in the adjacent farms, but only 3.4% in the free farms. The difference of the positive percentages between the affected and the free farms was statistically significant (P<0.05).