Detection of Salmonella DNA in Chicken Embryos and Environmental Samples by Polymerase Chain Reaction

Lawrence M. TUCHILI; Hiroshi KODAMA; Ravindra N. SHARMA; Ichiro TAKATORI; Girja S. PANDEY; Swithin KABILIKA; Masafumi MUKAMOTO; Sachiyo TSUJI; Tsuyoshi BABA

doi:10.1292/jvms.58.881

Lawrence M. TUCHILI, Hiroshi KODAMA, Ravindra N. SHARMA, Ichiro TAKATORI, Girja S. PANDEY, Swithin KABILIKA, Masafumi MUKAMOTO, Sachiyo TSUJI, Tsuyoshi BABA

Author information

Lawrence M. TUCHILI
Hiroshi KODAMA
Ravindra N. SHARMA
Ichiro TAKATORI
Girja S. PANDEY
Swithin KABILIKA
Masafumi MUKAMOTO
Sachiyo TSUJI
Tsuyoshi BABA
Author's Organization：
The School of Veterinary Medicine, University of Zambia
Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
The School of Veterinary Medicine, University of Zambia
The School of Veterinary Medicine, University of Zambia
The School of Veterinary Medicine, University of Zambia
The School of Veterinary Medicine, University of Zambia
Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University
Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University

Keywords: chicken embryo, DNA detection, polymerase chain reaction, Salmonella

JOURNAL FREE ACCESS

1996 Volume 58 Issue 9 Pages 881-884

DOI https://doi.org/10.1292/jvms.58.881

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Abstract

By polymerase chain reaction (PCR) using a pair of primers specific for Salmonella phoE gene a 365-bp specific gene fragment could be amplified from yolk of infertile eggs and dead-in-shell chicken embryos, and from environmental samples. Out of 45 dead-in-shell embryo samples, 20 (44.4%) were found positive for Salmonella DNA by PCR compared to 11 (24.4%) by bacteria isolation. Salmonella DNA could also be detected from infertile eggs, chicken faeces, floor litter and chick fluff, which incidence was higher than that by bacteria isolation.

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