Bacterial Diversity in Biological Filtration System for the Simultaneous Removal of Arsenic, Iron and Manganese from Groundwater

Rajan THAPA CHHETRI; Ichiro SUZUKI; Takuya FUJITA; Minoru TAKEDA; Jun-ichi KOIZUMI; Yoko FUJIKAWA; Atushi MINAMI; Tatsuhide HAMASAKI; Masataka SUGAHARA

doi:10.2965/jwet.2014.135

原著論文

Bacterial Diversity in Biological Filtration System for the Simultaneous Removal of Arsenic, Iron and Manganese from Groundwater

Rajan THAPA CHHETRI, Ichiro SUZUKI, Takuya FUJITA, Minoru TAKEDA, Jun-ichi KOIZUMI, Yoko FUJIKAWA, Atushi MINAMI, Tatsuhide HAMASAKI, Masataka SUGAHARA

著者情報

Rajan THAPA CHHETRI
Department of Chemical and Energy Engineering, Graduate School of Engineering, Yokohama National University, Japan
Ichiro SUZUKI
Department of Chemical and Energy Engineering, Graduate School of Engineering, Yokohama National University, Japan
Center for Risk Management and Safety Sciences, Yokohama National University, Japan
Takuya FUJITA
Department of Chemical and Energy Engineering, Graduate School of Engineering, Yokohama National University, Japan
Minoru TAKEDA
Department of Chemical and Energy Engineering, Graduate School of Engineering, Yokohama National University, Japan
Jun-ichi KOIZUMI
Department of Chemical and Energy Engineering, Graduate School of Engineering, Yokohama National University, Japan
Yoko FUJIKAWA
Research Reactor Institute, Kyoto University, Japan
Atushi MINAMI
Department of Human Life and Environment, Faculty of Human Environment, Osaka Sangyo University, Japan
Tatsuhide HAMASAKI
Department of Human Life and Environment, Faculty of Human Environment, Osaka Sangyo University, Japan
Masataka SUGAHARA
Department of Human Life and Environment, Faculty of Human Environment, Osaka Sangyo University, Japan

キーワード: arsenic, biological filtration, Leptothrix

ジャーナルフリー

2014 年 12 巻 2 号 p. 135-149

DOI https://doi.org/10.2965/jwet.2014.135

詳細

抄録

Bacterial diversity of the microbial consortia in a biological filtration pilot plant for the simultaneous removal of arsenic (As), iron (Fe) and manganese (Mn) from groundwater was analyzed. PCR-based denaturing gradient gel electrophoresis (DGGE) of bacterial 16S ribosomal RNA (rRNA) genes represented at least 6 dominant signals and many weak signals. Phylogenetic analysis using the nucleotide sequences of the 16S rRNA gene clone library constructed from the pilot plant sample showed the presence of the bacteria closely related to Gallionella and Leptothrix, which are supposed to be involved in the production of Fe and Mn oxides utilized for adsorbents of As in this system. On the other hand, aoxB gene was not detected, suggesting that arsenite-oxidizing bacterium would not be involved in the As removal of the pilot plant. These results indicated that the simultaneous removal of As, Fe and Mn from groundwater was conducted by the physicochemical sorption of As by the biogenic Fe and Mn oxides produced by the bacteria closely related to Gallionella and Leptothrix included in the microbial consortia of the pilot plant.

責任著者(Corresponding author)

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