Journal of Japan Water Works Association
Online ISSN : 2435-8673
Print ISSN : 0371-0785
Detection of Geosmin Biosynthesis Genes in Cyanobacteria in Raw Water
Kenji TAIRARumiko YANO
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2019 Volume 88 Issue 12 Pages 3-9

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Abstract

A musty odor compound, geosmin, causes problems of water treatment at water purification plant (WPP). To remove the odor, a large amount of powdered activated carbon is needed every year. The compound is produced by some kinds of filamentous cyanobacteria, genus Anabaena (Dolicospermum), inhabit in the water source. Usually, by monitoring cell numbers of the Anabaena with microscopy and geosmin concentration with solid phase micro extraction gas chromatography mass spectrometry (SPME-GC/MS) analysis, we determine how much amount of powdered activated carbon is needed to dose. In 2013, from the result of the experiment with microscopy, geosmin-producing Anabaena increased in two peaks for 3 months. During the second peak period, the geosmin concentration per one Anabaena cell was low level, about 4% of that of the 1st peak. It seemed that there was a possibility the cause of the difference of the geosmin concentration between the two peaks relates to a geosmin synthesis gene in cyanobacteria. To study a PCR detection method on raw water at WPP, we constructed two primers, 16SANB primer and GE1 primer, and had experiment of PCR amplifications with those primers. 16SANB primer is based on 16S rRNA gene in Anabaena and as the PCR reference gene. GE1 primer is based on geosmin synthesis operon gene and as the PCR target gene. From the results of the experiments, we could confirm that this detection method can be used practically.

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