Extraction Characteristics of Lysozyme Using Sodium Bis (2-ethylhexyl) Sulfosuccinate-Long Chain Alkyl Amines Mixed Reverse Micellar System

Abstract

In a mixed micellar systems of sodium bis (2-ethylhexyl) sulfosuccinate and di-2-ethylhexylamine or di-n-octylamine, the extraction of lysozyme is investigated by the phase transfer method. The extraction of lysozyme occurs at pH range between its pI and the pH where the extraction of water is observed. Lysozyme extracted into the mixed micellar systems can be successfully back-extracted with high activity yield.