Pyrogen Removal Using Fixed Bed of Immobilized Histidine

Abstract

Pyrogen removal using a fixed bed of immobilized histidine was investigated. An aqueous solution containing endotoxin (20 ng/cm³) was used for this study.
The breakthrough curve of pyrogen was not clear, but a practical breakpoint was obtained. The concentration of pyrogen in effluent and the adsorption capacity depended on ionic strength. When the ionic strength was less than 0.11 mol/dm³ and the flow rate was 0.021 cm/s, the concentration of pyrogen in the effluent was less than 0.1 ng/cm³.
In addition, when the bed was compressed the concentration of pyrogen in the effluent decreased and the adsorption capacity increased, but the pressure drop also increased. From these results, the favorable compression ratio was found to be about 0.1.