Removal of Pyrogen Using Immobilized Histidine by Batchwise Method

Abstract

The removal of pyrogen using immobilized histidine by a batchwise method was investigated. The pyrogen adsorption depended on ionic strength and was influenced by the shaking speed. The concentration of pyrogen in liquid phase decreased to less than 0.1 ng/cm³ from 20 ng/cm³ by batchwise adsorption with the immobilized histidine when the ionic strength was lower than 0.10 mol/dm³. This indicates that pyrogen could be removed practically by batchwise adsorption.