Establishment of a Simple Separation Process for Highly Pure β-Carotene from Cultured Carrot Cells

Abstract

β-Carotene sample prepared by the previously reparted separation process from cultured carrot cells was found to be pure as a carotenoid pigment but to contain some other lipids. We then improved the separation process by omitting the saponification process and improving the adsorption chromatography and liquid-liquid extraction. This new process markedly reduced the operation time, and highly pure β-carotene sample which contained no other lipid was obtained while maintaining the previous β-carotene yield (5.14μg/g-dry cells)