1991 Volume 17 Issue 3 Pages 642-648
Using a perfusion culture with bleeding, dense cultivation of mouse-mouse hybridoma cells was carried out in serum -free medium under glucose -limiting conditions. The effects of glucose concentration on cell growth and metabolic rates of carbon sources were investigated.
The specific growth and death rates were not influenced by glucose concentration, but the metabolism of glucose and glutamine was found to be remarkably affected by glucose concentration. High glucose concentrations resulted in both overutilization of glucose and high yields of lactate from glucose. Conversely, high ammonia accumulation was observed when glucose concentration in the broth was kept very low. The above results suggest that there exists an optimum glucose concentration for reducing specific lactate and ammonia production rates.
Cell growth was found to be well correlated with ATP production calculated from glucose and glutamine metabolisms. This means that the energy required for cell growth was constant in spite of varied metabolic behaviour. Hybridoma cells could adapt to glucose -limiting conditions and metabolize the alternative energy substrate of glutamine.
