Effect of Several Alcohol Additions on Backward Extraction of Lysozyme in Reversed Micellar Extraction

Abstract

The effects of several alcohol additions on backward extraction of lysozyme through the reverse micellar extraction were investigated. The reverse micellar phase was a 50 mol/m³ AOT/isooctane containing lysozyme in a concentration of 1 kg/m³ and the aqueous phase was a 1 kmol/m³ KCl solution at pH 11.5 containing the alcohol. There was little difference in the percent backward extraction with kind of alcohol, but the effect for accelerating backward extraction rate became large in the order of ethanol, isopropanol and 2-methyl-2-propanol.