Abstract
In this review on preparation methods of specific affinity ligands for separation and detection, examples of fusion proteins containing affinity tags for specific separation and also of selection methods of monoclonal antibodies from combinatorial libraries are summarized. Advantages of anti-peptide antibodies for immunoaffinity separation of proteins are discussed. Anti-peptide antibodies against peptides in N-or C-terminal regions of target proteins adsorb native proteins, as well as antigen peptides, and the adsorbed proteins ere specifically eluted from the antibodies by the eluent containing the antigen peptides, under mild conditions.
