リアルタイムPCR を用いたAeromonas hydrophila 壊死性軟部組織感染症の迅速診断

抄録

We report a case of rapidly progressive necrotizing soft tissue infection and sepsis followed by a patientʼs death. We suspected Vibrio vulnificus infection because the patientʼs underlying disease was cirrhosis and the course extremely rapid. No microbe had been detected at death. We extracted DNA from a blood culture bottle. SYBR green I real-time PCR was conducted but could not detect V. vulnificus vvh in the DNA sample. Aeromonas hydrophila was cultured and identified in blood and necrotized tissue samples. Real-time PCR was conducted to detect A. hydrophila ahh1, AHCYTOEN and aerA in the DNA sample extracted from the blood culture bottle and an isolated necrotized tissue strain, but only ahh1 was positive. High-mortality in necrotizing soft tissue infections makes it is crucial to quickly detect V. vulnificus and A. hydrophila.We found real-time PCR for vvh, ahh1, AHCYTOEN,andaerA useful in detecting V. vulnificus and A. hydrophila in necrotizing soft tissue infections.