1973 年 47 巻 4 号 p. 111-115
It has been popularly circulating in half legendary way among laymen that those who habitually eat garlic would rarely catch cold in winter time. The authors took it up rather seriously and undertook experiments to see the effects of garlic extracts against infections with influenza and Japanese encephalitis viruses in mice.
Viruses used were influenza virus AO/PR 8 strain, Japanese encephalitis virus Nakayama strain and JaGAr Ol strain. Mice used in the case of influenza were ddYS strain weighed 15g, in Japanese encephalitis, the same strain weighed 8-10g regardless the sex. Garlic extracts were prepared by alcohol extraction using low percent alcohol. The extracts were administrated per os to the mice. Further, to see the effects of the combination of garlic extracts and vitamines and liver extracts, we prepared the following solutions as: Solution A-garlic extracts without any additives; Solution B-garlic extracts plus VB1; Solution C-garlic extracts plus VB12; Solution D-garlic extracts plus VB1 and VB12; Solution E-garlic extracts plus VB1, VB12 and liver extracts. Infections of influenza and Japanese encephalitis were made by pernasal and intracerebral inoculations, respectively. The inoculated mice were observed for three weeks. LD50 was measured by Reed and Muench method. Influenza infected mice were all autopsied and the consolidation of the lungs was comparatively observed.
The results were summarized as follows:
1. In group in which daily administrations of the solutions were begun 15 days previous to pernasal influenza virus inoculations, the effects were significant. In our data, solution A and E were best. In group in which the solutions were begun being given at the same time to the virus inoculations, the effects were hardly appreciable.
2. In Japanese virus inoculated cases, the results were against our expectation. Any solution given even 15 days previously to the inoculations displayed no effect at all.