Studies on Rubella I.
Rubella virus neutralization test by plaque count assay
1979 Volume 53 Issue 9 Pages 397-408
Details
1979 Volume 53 Issue 9 Pages 397-408
There are several assays for detection of antibody of rubella virus. The comparison of the plaque containing neutralization test (NT) and the hemaggulutination test (HAI) was studied in 59 paired sera from school boys and girls, which were obtained during the period of outbrake of rubella, and in the commercially availlable positive and negative sera for the HAI.
The results are as follows.
1. Established rabbit kidney cell line (RK-13c, Chiba Serum Inst., Japan) was superior to RK-13F (Flow Co., U. S. A.) for plaque count NT assay to rubella.
2. Optimum plaque formation occured at 30°C.
3. NT titers of the standard sera which contained IgG fraction, were increased by adding guinea pig serum as complement.
4. Straight line, y=x, was obtained when HAI and NT titers on clinical specimens were compared ±
5. Very low levels of antibody were found with NT assay in some specimens in which HAI was below 1: 8.
