Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
ISSN-L : 0387-5911
Clinical and Bacteriological Study on Microbial Flora in the Vagina
(2) A Study on Isolation Rates of Group B Streptococci in the Vagina of Pregnant and Non-pregnant Women
Hirofumi OHASHI
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JOURNAL FREE ACCESS

1981 Volume 55 Issue 2 Pages 109-115

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Abstract

This study was done to assess the incidence of group B streptococci (GBS) in the vagina of pregnant and non-pregnant women in further detailed classification into the stage of pregnancy, the phase of menstrual cycle and age groups. On cases of GBS-positive, their serotype studies were also done.
The subjects of the study were the 301 outpatients (177 pregnant and 124 non-pregnant women) who visited the Department of Obstetrics and Gynecology at the Central Hospital of Japanese National Railways between April 1980 and July 1980.
Vaginal specimen was obtained from each woman with sterile cotten-tipped swab that was inserted into the vagina and rubbed against the vaginal wall. The specimen was inserted into a small sterile test tube containing the sterile silicagel for transportation to the laboratory. It was placed and enriched in Pike medium and then the culture was inoculated onto a Heart Infusion medium containing 5% debrinated sheep blood. GBS was identified by the form of the colony, hemolysis, bacitracin sensitivfiity test and Phadebact Streptococcus Test for grouping, and then was examined for serotyping using the anti-sera prepared by Kanagawa Prefectural Institute.
The GBS positive rate of pregnant women was 13.6%, with no variation of the rate by the stage of pregnancy. That of nonpregnant women was 21.8% with no variation of the rate by the phase of menstrual cycle.
A tendency of increase of GBS positive rate by age was observed, and GBS positive rates of postmenopausal women (35.3%) is higher than that of premenopausal women (19.6%).
The most frequent serotypes from pregnant women were type III (37.5%), Ia (25.0%), Ic (16.7%), but type Ib, R, IIIR were isolated less frequently. The most frequent serotypes from non-pregnant women were type Ia (40.7 %), Ic (22.2 %), R (11.1%), but type Ib, III, IIIR were isolated less frequently. Except this difference that many type III cases were seen among pregnant women and less among nonpregnant women (P<0.05), no particularly characteristic finding were not made.
No statistically significant differences have been shown in the serotypes of GBS during the stage of pregnancy.

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© The Japansese Association for Infectious Diseases
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