Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
ISSN-L : 0387-5911
Relationship between Intestinal Bacterial Flora and Infection with Intestinal Parasites in Mice
(I) Effect of Humoral Immunity in C57BL/6 mice Infected with Nippostrongylus brasiliensis
Atsuo HIRABAYASHIKeizo NAGASETetsuzo TOTANI
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1983 Volume 57 Issue 7 Pages 551-562

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Abstract

The host-parasite relationship between intestinal bacterial flora and infection with intestinal parasites was immunologically studied by the immunoenzyme technique in C57BL/6 germfree mice infected with N brasiliensis.
Canges of immunoglobulin-producing cells in a mucosal bassel layer was examined by the immunoenzyme technique. The result revealed that developments of systemic and regional immune systems was suppressed in the germfree mice, though an intestinal bacterial flora affects the host's immune system. Immunoglobulin-producing cells differed in number for the conventional and germfree groups respectively. Most of those cells were observed in a mucosal bassel layer near the villus base of the intestinum tenue and on a ductal surface of a mucosal basel layer of the intestinum crassum. In the gremfree group compared to the conventional group, IgA-producing cells significantly decresed in the number, while IgG and IgM-producing cells decresed slightly.
Since immunoglobulin-producing cells were not greatly affected among the group infected with N brasiliensis little effects were observed in an infection with intestinal parasites and an intestinal bacterial flora seemed to play a major role.
The plaque method by Jerne was applied in order to examine reaction of SRBC. In conventional group, large amounts of IgM-PFC and IgG-PFC were observed in aged mice. A similar tendency was observed among the infected groups. In the germfree group, an obvius increse was seen compred to the conventional group.
These results suggested that the possibility of immunoglobuline producing plasma cells was preserved in the germfree mice.
The amount of Serum-IgA indicated a tendency to increase when a mouse survied for additional weeks and it was affected by infection. The amount was generally low for the germfree group in comparison with the conventional group. IgG increased in amount for the conventional group when a mouse surived for additional weeks, indicating the obvious effects of infection. Differences, caused apparently by infection, were observed among the aged and infected mice of the germfree group. An increase in IgG was observed for the germfree, infected and aged group. IgM was in higher in amount than 10 week-old. It dropped when the mice became 20 week-old. The infected group indicated a higher rate for all markers than the contrast group. Among the germfree groups, the infected group indicated a higher rate for the all markers.

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© The Japansese Association for Infectious Diseases
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