1985 Volume 59 Issue 3 Pages 266-276
On the course of treatment for children with malignancies, we have often experienced so-called sepsis like symptomes. From these cases, it is very difficult to detect pathogenic microorganisms by conventional blood culture methods. In this report, I discussed about the Lysis-Filtration blood culture technique which was first described by Zierdt et al. and I tried to reform some important points.
The following results were obtained.
1) The optimal condition of Lysing Solution (L.S.) was determined for both bacterial toxicity and lysing capability. The L.S. was satisfied by the condition with concentrations ranging from 0.05 to 1.0% of Protease, ranging from 0.1 to 2.0% of Tween 20 and ranging from 6.0 to 10.0 of pH.
2) There were no specific changes in the nature of L.S. when stocked at 4°C for one year.
3) As the antibactericidal factors of the blood are removed before cultibation, the detected CFU of bacteria was correlated with inoculated blood volume. This is the reason why L-F method was more effective as the lower level bacteremia with less than 1 CFU/ml.
4) The L-F method is higher than the broth method regarding the detection rate of bacteria in artificial bacteremia by S. aureus (p<0.05). When the bacterial level was less than 1 CFU/ml, the significance was getting higher (p<0.01). At the artificial bacteremia by E. coli, the same tendency was recognized.
5) Both culture methods were used at the same time for the patients of malignancies who had febrile episodes. 9 cases of 163 (5.5%) L-F method and 8 cases of 162 (4.9%) conventional method detected bacteria, but no significance was recognized between the detective rate of both ways.
