1985 Volume 59 Issue 6 Pages 597-604
The roles of oxidative metabolism and lysosomal enzymes in the phagocytosis of activated macrophages against Pseudomonas aeruginosa, Staphylococcus aureus, and latex particles were studied. It can be said that enhancement of the host defence mechanism against p. aeruginosa infection was increased by activating the reticuloendothelial system by Propionibacterium acnes pretreatment. The oxidative metabolism as measured from cheniluminescence, and lysosomal enzymes against p. aeruginosa play an important role in the intracellular killing of bacteria, and these activities are enhanced by p.acnes treatment. Activated macrophages generated the highest chemiluminescence response when they ingested p.aeruginosa in combination with gentamicin and gamma-globulin. In contrast, such mechanisms show little participation against S. aureus and latex particles.