Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
ISSN-L : 0387-5911
Detection of Serum Candida Antigen by an Enzyme-Linked Immunosorbent Assay with Avidin-Biotin System (2)
Hiroshi SUZUKIAkira NAKAMURAMakoto MIYAJI
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1987 Volume 61 Issue 1 Pages 1-8

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Abstract

In a previous paper, we presented a basic study of an enzyme-linked immunosorbent assay with avidin-biotin system (A-B ELISA) for the detection of serum Candida antigen. In this report, the usefulness of A-B ELISA was evaluated in experimental systemic candidiasis of rabbits and patients with candidiasis.
The serum Candida antigen was detected from the first day until death (7 days after inoculation) in a fetal rabbit. However, in non-fetal rabbits, the antigen was detected only on the early stage of infection.
In clinical cases, the antigen was detected in 5 patients with candidiasis by the assay. They all were compromised hosts, neonates 2, acute lymphocytic leukemia 2 and one suspected of acute myelofibrosis.
Blood samples from the 3 patients grew Candida. In one of them, the antigen had been detected while C. albicans was recovered from her blood. In another case, the antigen was detected persistently even after C. tropicalis disappeared from her blood due to a treatment with antifungal agents.
Candida was not recovered from blood samples of the other two. However, we succeeded to detect the antigen from them. Judging from this result in addition to their clinical features and laboratory data, they were suspected of systemic candidiasis. When they were treated with antifungal agents, their clinical symptoms and laboratory findings were improved, and antigen disappeared.
These results seem to suggest that A-B ELISA is useful for the early diagnosis of systemic candidiasis.

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© The Japansese Association for Infectious Diseases
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