Investigation About Application of Serum-Free Media to HIV Study

Abstract

In order to apply serum-free meida to the HIV study, the effects of serum-free media in chronically HIV-1 infected cell growth, in HIV-1 replication and in RTA were studied for detecting the virus.
1) all the serum-free media gave good cell growth compared with the RPM-1640 supplemented with 10% FCS.
2) HIV-1 replicated more in lower protein content serum-free media than in higher protein content serum-free media and in RPM-1640 supplemented with 10% FCS.
3) HIV-1 RT activity was reduced by FBS in proportion to the concentration. Serum-free media showed no reduction of the activity compared with high concentrations of FCS.
These data indicate that serum-free media are useful for HIV study in vitro.