Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
ISSN-L : 0387-5911
Detection of Verotoxin-Producing Escherichia coli Using Polymerase Chain Reaction from Dairy Cattles.
Hiroshi TADASachiko ITAMIYasuo YAMAMOTOKazuhiro KOBAYASHIMasumi TAGUCHIMuneo NAKAZAWA
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1992 Volume 66 Issue 10 Pages 1383-1389

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Abstract

The vero cytotoxin (VT) is responsible for hemorrhagic colitis and hemolytic uremic syndrome. Polymerase chain reaction (PCR) was used to detect VT-producing coliform bacteria from dairy cattle. It was found that 39 (33.3%) of the 117 fecal samples examined were recognized with VT genes in BGLB enrichment broth by the PCR method (named BGLB-PCR). Of the VT-positive samples, 31 samples (26.5%) were found to have VT-producing Escherichia coli. Frequencies of isolation in younger cattles (under 5 months) were 31.3-32.9%. On the other hand, the PCR method using the bacterial suspension of some colonies from DHL selective isolation medium (named DHL-PCR), was used for 105 samples. The DHL-PCR was validated according to the number of colonies tested for detecting VTEC. When using E. coli strains which have been stored after isolation by the conventional culture method, the VT-producing strains found were 7 (10.3%) of the 68 isolates tested. The 101 out of the 108 VTEC strains from cattle were classified into 14 0 groups. 4 0 serogroups (026, 0111, 0145, 0157) from 60% of VTEC positive cattle, were also the most common in humans with diarrhea. All E. coli O157: H7 isolates failed to ferment after 48 hrs and to hydorolyze 4-methyl-umbelliferyl-beta;-D-glucuronide (MUG). These results suggests that cattle may play an important role in human VTEC infections. The BGL B-PCR technique is usefull in ecological studies for VT-producing pathogens.

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© The Japansese Association for Infectious Diseases
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