Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
Continuous In Vitro Culture of Plasmodium falciparum Using Microaerophilic Gas Generators and Portable Incubator
Tomoko ONDAQinghua LINShigeyuki KANOMamoru SUZUKI
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1999 Volume 73 Issue 11 Pages 1095-1098

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Abstract

AnaeroPack® Malaria Culture System (SUGIYAMA-GEN Co., Ltd.) using AnaeroPack®·plas (5%O2, 5%CO2) and AnaeroPack®·CO2 (15%O2, 6%CO2) was evaluated by comparing with the standard laboratory in vitro continuous culture technique. Two culture-adapted strains of Plasmodium falciparum, SGE-1 (chloroquine sensitive strain) and K1 (chloroquine resistant strain), were continuously cultured for 26 days in vitro under the 3 systems. The parasite proliferation curves under the different set systems were paralleled in both strains, which demonstrate that this AnaeroPack® Malaria Culture System is useful for the culture-adapted strains of P. falciparum. Although further test using isolates from falciparum malaria patients should be carried out, the AnaeroPack® Malaria Culture System seems promising for the culture in the field studies.

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© The Japansese Association for Infectious Diseases
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