2001 Volume 75 Issue 4 Pages 333-340
From October 1998 to April 1999, 72 stool specimens from patients of acute gastroenteritis werecollected in a pediatric clinic in Urawa city, Japan and were examined for the detection of SRSV byelectron microscopy and reverse transcription-polymerase chain reaction (RT-PCR). Forty-eight (67%) out of 72 were SRSV positives by both tests. In this season, a prevalence of SRSV peaked in December1998. Among reported clinical symptoms, vomiting was reported more frequently than theother symptoms, such as diarrhea and fever. Furthermore, the diarrhea was intense among childrenunder 1 year old. For the detection of SRSV by RT-PCR, we used two kinds of primer sets (MR3/MR4 and P1/P2/P3). The P1/P2/P3 primer was most sensitive for the detection of SRSV.
