Cefotaxime-Resistant Shiga Toxin-Producing Escherichia coli O26: H11 Isolated from a Patient with Diarrhea

Abstract

A shiga toxin-producing Escherichia coli (STEC) 026 strain resistant to cefotaxime (CTX) and cefpodoxime (but not ceftazidime) was isolated from the faecal sample of a 17-year-old outpatient with diarrhea.
The double disk synergy test, twin test, polymerase chain reaction and sequence analysis confirmed that the strain produced CTX-M-3 type extended-spectrum β-lactamase (ESBL).
Conjugation experiment results suggested that the CTX resistance in this strain was determined by an approximately 85kbp plasmid that was readily transferable to a susceptible recipient E. coli strain.
This is the first report from Japan of CTX-M-3type ESBL-producing STEC O26.