肩関節
Online ISSN : 1881-6363
Print ISSN : 0910-4461
ISSN-L : 0910-4461
骨髄由来間葉系幹細胞を用いた腱板再生について-PGAシートをscaffoldとして-
横矢 晋望月 由大前 博路菊川 和彦柏木 健児夏 恒治三原 修三今田 岳男白川 泰山奥平 信義安達 長夫
著者情報
ジャーナル フリー

2006 年 30 巻 3 号 p. 449-455

詳細
抄録

In irreparable rotator cuff repair we usually perform patch graft by using autologous femoral fascia or a polytetrafluoroethylene sheet. However, these methods have problems regarding sacrifice of normal self-tissue and foreign body reaction.
Therefore, development of a method regarding rotator cuff regeneration by tissue engineering technique was expected. We reported on an experimental study about rotator cuff regeneration by using a polyglycolic acid (PGA)sheet as a scaffold with bone marrow-derived mesenchymal stem cells. We made a defect of the infraspinatus tendon insertion in 36 shoulders of Japanese white rabbits and filled the defect with poly-lactide caprolactone (PLC) (PLC group), PGA sheet (PGA group), and PGA sheet with cultured mesenchymal stem cells (MSC group). We sacrificed them at 4,8 and 16 weeks after operation, and evaluated them histologically with H-E, Safranin O, and Azan stain and type I and III collagen immunohistochemical stain. In the PLC group, we found massive foreign body reaction around the PLC fibers although they were absorbed gradually, and the cell invasion into the fibers was a little. The arrangement of cells and fibers were irregular and the cartilage formation in insertion was not found. In the PGA group, PGA fibers were almost absorbed at 16 weeks and regenerated fibrous tissues showed the crimp pattern. In insertion we found the 4 layer cartilage pillar pattern but mainly consisted of type III collagen. In the MSC group, we found the 4 layer cartilage pillar pattern regularly in insertion at 8 weeks and more type I collagen was found than type III at 16 weeks. Therefore, rotator cuff regeneration using a PGA sheet with bone marrow derived mesenchymal stem cells had a good capacity of regenerating tendon-bone insertion and producing type I collagen. We thought this method quite useful for regeneration of a rotator cuff defect clinically.

著者関連情報
© 日本肩関節学会
前の記事 次の記事
feedback
Top