Abstract
Two protein quantitation methods for measurement of cell growth in tissue culture were examined on their utility by using L 929 cell line. One was a modified Lowry method using a phenol reagent, and the other was the Dye binding assay method described by Bradford. The range of cell protein measurement was found to be enough to use and a little wider by the modified Lowry method than by the Dye binding assay method ; 5∿100μg by the former and 5∿60μg by the latter. The calculated factor to convert bovine serum albumin standard equivalent (μg) to cell count was 2.0×10^3 cells by the modified Lowry method, and 2.8×10^3 cells by the Dye binding assay method. Furthermore, those two methods were compared with other two methods to which protein quantitation technique was not applied ; one was nucleus counting through a microscope, and the other was a kind of colorimetric determination of the stained cell layer with Monocellater. Both of the protein quantitation methods were confirmed to give much less amount of scatter in measured values than nucleus counting or than the method with Monocellater.
