KENBIKYO
Online ISSN : 2434-2386
Print ISSN : 1349-0958
Feature Articles: High-resolution Structural Analysis of Biological Macromolecules
High Resolution Structural Analysis of the Dynein-Microtubule Complex
Keiko HiroseHironori Ueno
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JOURNALS FREE ACCESS

2009 Volume 44 Issue 2 Pages 93-97

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Abstract

Dynein is a molecular motor that moves along a microtubule. Dynein interacts with a microtubule using the microtubule-binding domain at the tip of its stalk, and a predominant model for the movement involved a rotation of the stalk relative to microtubules. However, the stalk, which is a single coiled-coil, was difficult to observe in the microtubule-bound state, either by negative staining or by cryo EM. Here, using a new method “cryo-positive stain EM”, which is a combination of uranyl acetate staining and cryo EM, we succeeded in observing microtubule-bound stalks. Contrary to the above model, the stalks did not change angles relative to microtubules between the two nucleotide states we observed.

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© 2009 The Japanese Society of Microscopy
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