KENBIKYO
Online ISSN : 2434-2386
Print ISSN : 1349-0958
Feature Articles: Biological Applications of STEM Tomography
Quantitative Determination of Beam Irradiation Damages to Biological Specimens Caused by STEM Tomography
Ai HiraseAtsuo Miyazawa
Author information
JOURNALS FREE ACCESS

2009 Volume 44 Issue 4 Pages 248-251

Details
Abstract

Until now, Scanning transmission electron microscopy (STEM) tomography has not yet become popular in the biological research field. It is widely considered that the beam damage of STEM would be very severe, since a convergent electron beam is required for the imaging. On the other hand, STEM allows us taking tilt series of images from thick biological specimens (up to 1μm) without using ultra-high voltage electron microscopy for finding out 3-D reconstruction of the specimens. To demonstrate a benefit of STEM tomography for the analysis of biological specimens, the shrinkage of the specimens was measured for the quantification of the beam irradiation-induced damage. The difference of the size of intracellular organelles, such as mitochondria was determined, before and after taking the tilt series of images for tomography. In the result, it was appeared that the beam irradiation-induce damage of STEM tomography was considerably less than that of TEM tomography.

Information related to the author
© 2009 The Japanese Society of Microscopy
Previous article Next article
feedback
Top