KENBIKYO
Online ISSN : 2434-2386
Print ISSN : 1349-0958
Feature Articles: Biological Applications of STEM Tomography
Structural Analysis of Cell Membranes by Small Convergence Angle HAADF-STEM Tomography
Nobuhiro MoroneKazuhiro Aoyama
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2009 Volume 44 Issue 4 Pages 257-261

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Abstract

The rapidly-frozen and deeply-etched EM image allows us to find that the cytoplasmic surface of the plasma membrane has a large undulation covered with the networks of caveolae, clathrin-coated pits, and actin-based membrane skeletons. In this paper, we compared the three-dimensional reconstruction of the cellular freeze-replica containing of various focal point distances, between the conventional TEM tomography and the variable convergence-angle STEM tomography, by using of the electron microscope equipped with the three condenser-lens system containing Cs correctors. It was clearly found that the small convergence-angular (0.5mrad) HAADF-STEM tomography could make the best 3D reconstruction with little computational artifacts through the missing wedge during EM observation. This proposed freeze-etch tomography could be expected as a novel tool for the plasma membrane research.

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© 2009 The Japanese Society of Microscopy
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