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Online ISSN : 2434-2386
Print ISSN : 1349-0958
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Atmospheric Scanning Electron Microscope Observes Cells in Solution
Hidetoshi NishiyamaMitsuo SugaToshihiko OguraYuusuke MaruyamaMitsuru KoizumiKazuhiro MioShinichi KitamuraChikara Sato
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2009 Volume 44 Issue 4 Pages 262-267

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Abstract

We have developed an inverted scanning electron microscope with a detachable, open-culture dish, capable of 8nm resolution, and combined with a fluorescence microscope quasi-simultaneously observing the same area. In this system, cells or organelles are observed by the fluorescence microscopy from the top, and by the high resolution scanning microscopy from the bottom. Cells cultured on the open dish can be externally manipulated under an optical microscopy, fixed, and observed using scanning electron microscopy.

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© 2009 The Japanese Society of Microscopy
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