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Cellulose Nanofibers in Water Observed by the Quick-Freeze, Deep-Etch Replication and HAADF-STEM
Kazunori KawasakiTomoki KatoYuka WatanabeKoichi UegakiKenichi HarashimaKota OguraMinoru HayashidaKazuhiko Ishikawa
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JOURNALS FREE ACCESS

2010 Volume 45 Issue 1 Pages 60-64

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Abstract

To apply cellulose as an industrial resource, a novel method for pulverization of crystalline cellulose by water-jet was developed. Morphology of cellulose treated with the method was examined by electron microscopic observations. The quick-freeze, deep-etch replica technique combined with high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) and electron tomography was useful to visualize the fine structures of cellulose in water. The result demonstrated that the treatment dispersed cellulose to nanofibers (20-40nm wide) which formed three-dimensional networks in water.

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© 2010 The Japanese Society of Microscopy
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