KENBIKYO
Online ISSN : 2434-2386
Print ISSN : 1349-0958
Lectures
Reliable Method for Obtaining Serial Ultrathin Sections Using New Small Tools
Shigeo Kita
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JOURNALS FREE ACCESS

2011 Volume 46 Issue 4 Pages 253-257

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Abstract

Procedure of obtaining serial ultrathin sections for electron microscopy consists of several steps: cutting of uniform very thin sections by ultramicrotome; making ribbon sections for taking pictures in electron microscope one after another; separating ribbon sections to fixed length for mounting grids; and collecting ribbons on the grids with no failure. The present report describes a reliable method for obtaining serial ultrathin sections from a resin block of biological tissues using new small tools.

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© 2011 The Japanese Society of Microscopy
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