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Online ISSN : 2434-2386
Print ISSN : 1349-0958
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Live-Cell Imaging of Endocytosis in the Budding Yeast
Junko ToshimaJiro Toshima
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JOURNALS FREE ACCESS

2013 Volume 48 Issue 3 Pages 209-212

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Abstract

The budding yeast Saccharomyces cerevisiae is an excellent model organism with which to study intracellular trafficking pathways, such as the secretory and endocytic pathways. While “forward genetics”, which involves the identification of a gene responsible for a specific phenotype, is still useful in studies of yeast, recent advances in fluorescence microscopy have facilitated a “reverse genetics” approach for revealing the functions of genes by analyzing the phenotypes resulting from mutation. Here we introduce a technique for live-cell imaging of yeast endocytosis and analysis of the molecular mechanisms involved.

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© 2013 The Japanese Society of Microscopy
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