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Feature Articles: Real-Time Morphological Changes of the Living Bacterial Cells Are Revealed by Visualization...
Observation of Morphological Change of Bacteria by High-Speed Atomic Force Microscopy
—Antimicrobial Action of Propolis on Porphyromonas gingivalis
Satoru HirayamaYuri YoshimasuRyoma Nakao
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2019 Volume 54 Issue 2 Pages 72-76

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Abstract

Propolis is a resin-like natural product produced by mixing saliva with sap and pollen by bees and is also used in folk medicine for periodontal treatment. In this study, we examined the antimicrobial activity of propolis ethanol extract against Porphyromonas gingivalis, which is regarded as a keystone among periodontopathic bacteria. As a result, it was found that P. gingivalis showed high sensitivity to propolis, and propolis showed a bactericidal effect more effective than ampicillin. In addition, we observed non-immobilized live P. gingivalis cells using high-speed atomic force microscopy system BIXAM, and succeeded in observing in real-time how propolis induces abnormal membrane vesicle-like structures on the cell surface. These findings suggest that propolis has excellent selective antibacterial activity against P. gingivalis, and suggest its applicability to the prevention and treatment of periodontal disease.

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© 2019 The Japanese Society of Microscopy
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