The experiment which have been made clear the effect of turbulence caused by combustion analyzed in the second paper was done by this paper. The experiments let equivalence ratio of supplied air-fuel mixture, pressure, configuration of combustion chamber and location of ignition plug change using constant volume combustion chamber. As a result of experiments, it became clear that the turbulence caused by combustion was in proportion to the density of supplied air-fuel mixture and mean velocity of flame propagation. The empirical formula is shown next. u^^^'0≈0.058ρ0.440V0.40fm The calorific value calculated using this empirical formula became small around 8% value with mean than theoretical calorific value. And also it became clear that the dispersion of estimation was small comparatively because a value of correlation coefficient was 0.98.