The velocity field and the temperature distribution are measured simultaneously in a nonpremixed jet flame by applying particle image velocimetry (PIV) and planar laser induced fluorescence (PLIF) of nitric oxide with known concentration added as a tracer. The fuel flow of low temperature is surrounded by the high temperature region. Also, turbulence kinetics in a jet flame was analyzed based on the statistical characteristics of the temperature and velocity, including mean velocity, turbulence intensity and Reynolds stress. It was shown that u' is quickly decayed in the high temperature region. And the maximum point of density weighted average of Reynolds stress is located at fuel stream side of shear layer. As a result, production of the turbulence is mainly occurred in the fuel stream.