The standardization of a fatigue testing method is very important for collecting fatigue strength data for threaded fasteners. The Japanese Industrial Standard on this testing method was published by taking account of the ISO Standard and the JSME Standard. In the procedure of drafting the above JIS, the experimental research and statistical discussions were conducted and the main conclusions are as follows: (1) Three companies in the first-step experiment and six companies in the second-step experiment performed the fatigue tests, and no confusion resulted in the application of the 14 S-N testing method according to the JSME Standard. (2) Based on the 14 S-N testing method, the experiments of the one-way classification model and the two-way classification model were performed, and the method of analysis of variance for such experimental results is shown.