In emergency transportation by ambulance, a patient receives acceleration in various direction. Particularly, longitudinal or lateral acceleration in braking and curve driving causes patient's stress such as blood pressure variation in brain, vomiting or feeling of discomfort. To reduce such patient's stress, we developed the 2-degree-of-freedom (2 DOF) actively-controlled bed (ACB) that reduces the acceleration acting on a patient by controlling posture of the bed. This paper reports its structure, control scheme and control performance. The effect is examined by experiment in terms of cancellation rate of acceleration and ride quality. Using the actual driving data of ambulance, the cancellation rate of acceleration in emergency transportation is also estimated by simulation.