ESTABLISHMENT OF A NOVEL HUMAN LEUKEMIC CELL LINE, IEZ-3, WITH POSITIVE MEGAKARYOCYTIC MARKERS

Abstract

A leukemic cell line, IEZ-3, was successfully established from a ly8m old girl with a diagnosis of acute megakaryocytic leukemia. These cells were negative to peroxidase reaction, PAS stain, and esterase stains, just as the original leukemia cells were. Immunological study using monoclonal antibodies was positive to GP IIb/IIIa, suggestive of megakaryocytic origin. These findings show that IEZ-3 is a unique cell line established from a de novo leukemia, expressing megakaryocytic features.